A non-targeted metabolomics study on different glucose tolerance states

作者：Gu, Yan; Zang, Peng; Li, Li-qin; Zhang, Hui-zhi; Li, Ji; Li, Jin-xia; Yan, Yan-yan; Sun, Shu-mao; Wang, Jia; Zhu, Zhuang-yan^*

来源：International Journal of Diabetes in Developing Countries, 2019, 39(3): 478-485.

DOI：10.1007/s13410-018-0662-x

• Type 2 diabetes
• Impaired glucose regulation
• Metabolomics
• Gas chromatography
• mass spectrometry

A non-targeted metabolomics method was employed to study metabolic characteristics in subjects with different glucose tolerance. Plasma samples of 120 participants with normal glucose tolerance (NGT), impaired glucose regulation (IGR), and type 2 diabetes (T2D) were collected. Gas chromatography/mass spectrometry (GC/MS) was used to profile and compare the plasma metabolome among the three groups. Through the use of multivariate statistical analysis, we found distinct metabolome change from NGT to IGR and to T2D. ANOVA found that the IGR and T2D groups had perturbations of monosaccharide and lipid metabolism, disorders of glucogenic amino acids, and branched-chain amino acid catabolism. Furthermore, we also found that the levels of 2-hydroxybutyrate and 2-ketoisocaproate were progressively increased with glucose tolerance severity. The results from this study help us better understand the relationship between plasma metabolism and glucose tolerance states and also suggest that 2-hydroxybutyrate and 2-ketoisocaproate may be closely associated with the development of T2D.

• 出版日期2019-7
• 单位山西大同大学