A comparison of the accuracy of iTRAQ quantification by nLC-ESI MSMS and nLC-MALDI MSMS methods

作者：Shirran Sally L; Botting Catherine H^*

来源：Journal of Proteomics, 2010, 73(7): 1391-1403.

DOI：10.1016/j.jprot.2010.03.003

• iTRAQ quantification
• nLC-ESI MSMS
• nLC-MALDI MSMS
• Mascot
• ProteinPilot

The accuracy of quantification obtained using the iTRAQ labelling methodology for measuring protein ratios more extreme than 1 1 was investigated A comparison of nLC-ESI MSMS and nLC-MALDI MSMS analysis routes was performed A fixed concentration of a standard six protein mix was spiked with two proteins at a range of concentrations The two data analysis programmes, Mascot and ProteinPlot Paragon, were also compared Whilst the lower ratios could be measured accurately, greater discrepancies were seen for the higher ratios, particularly by nLC-ESI MSMS Filtering out the weaker reporter ion signals improved the accuracy of the ratios this is likely

• 出版日期2010-5-7