A new fluorescent probe for quick and highly selective detection of hydrogen sulfide and its application in living cells

作者：Ren, Yanshen; Zhang, Lulu; Zhou, Ziyan; Wang, Suilou^*; Xu, Yungen; Gu, Yueqing; Zha, Xiaoming^*

来源：New Journal of Chemistry, 2018, 42(16): 13884-13888.

DOI：10.1039/c8nj02178c

• TURN-ON PROBE
• SEQUENTIAL DETECTION
• REACTIVE OXYGEN
• H2S
• SULFUR
• DONORS
• SENSOR
• LIGHT
• SHIFT

Hydrogen sulfide (H2S) is one of the endogenous regulators of many physiological processes. NIPY-OH based on an imidazo[1,5-]pyridine has a large Stokes shift, high photostability with green-emission, relatively high fluorescence quantum yield. NIPY-OH could serve as an excellent candidate for the design of fluorescent probes. However, the reported probes cannot sufficiently take advantage of the optical properties of NIPY-OH due to the water-soluble problem. Herein, a new fluorescent probe NIPY-PBA, based on NIPY-OH, was designed for the detection of hydrogen sulfide. By rational design, compared with other probes, we successfully obtained the probe with higher sensitivity (limit of detection = 43 nM) and shorter response time (1.5 minutes) due to better water solubility. NIPY-PBA maintained the excellent optical properties of a 230-fold fluorescence enhancement with a large Stokes shift (208 nm) at the same time. Furthermore, NIPY-PBA was applied for the fluorescence imaging of H2S in living cells with low cytotoxicity, indicating NIPY-PBA has great potential to monitor H2S in biological systems.

• 出版日期2018-8-21
• 单位中国药科大学; 南京医科大学