Analysis of photosystem II biogenesis in cyanobacteria

作者：Heinz Steffen; Liauw Pasqual; Nickelsen Joerg; Nowaczyk Marc

来源：Biochimica et Biophysica Acta-Bioenergetics, 2016, 1857(3): 274-287.

DOI：10.1016/j.bbabio.2015.11.007

• Cyanobacteria
• Photosystem II assembly
• Thylakoid membrane
• SPR
• Membrane fractionation
• CX-MS

Photosystem II (PSII), a large multisubunit membrane protein complex found in the thylakoid membranes of cyanobacteria, algae and plants, catalyzes light-driven oxygen evolution from water and reduction of plastoquinone. Biogenesis of PSII requires coordinated assembly of at least 20 protein subunits, as well as incorporation of various organic and inorganic cofactors. The stepwise assembly process is facilitated by numerous protein factors that have been identified in recent years. Further analysis of this process requires the development or refinement of specific methods for the identification of novel assembly factors and, in particular, elucidation of the unique role of each. Here we summarize current knowledge of PSII biogenesis in cyanobacteria, focusing primarily on the impact of methodological advances and innovations. This article is part of a Special Issue entitled Organization and dynamics of bioenergetic systems in bacteria, edited by Conrad Mullineaux.

• 出版日期2016-3