A novel hairless mouse model for malignant melanoma

作者：Nguyen Dinh Thang; Yajima Ichiro; Nakagawa Kaoru; Tsuzuki Toyonori; Kumasaka Mayuko Y; Ohgami Nobutaka; Ly Thuy B; Iwamoto Takashi; Watanabe Daisuke; Kato Masashi^*

来源：Journal of Dermatological Science, 2012, 65(3): 207-212.

DOI：10.1016/j.jdermsci.2011.10.010

• MOBKL2B
• Melanoma
• Animal model
• Hairlessness

Background: An appropriate animal model for malignant melanoma could be a strong tool to develop biomarkers through analysis of melanomagenesis.
Objective: Development of a novel animal model that spontaneously develops malignant melanoma with a high percentage.
Methods: We crossed oncogenic RET (RFP-RET)-carrying transgenic mice of line 304/B6 (RET-mice) with hairless mice (hr/hr) and newly established hairless RFP-RET-transgenic mice of line 304-hr/hr (HL-RET-mice).
Results: The HL-RET-mice developed hyperpigmented skin and benign melanocytic tumors without exception. More importantly, 63.8% (46/72) of the benign tumors were transformed to malignant melanoma in the HL-RET-mice. Mean time until the development of benign melanocytic tumors (2.4 months; n = 102) in the HL-RET-mice was about half of that in the original RET-mice (4.6 months; n = 20). Mean life span in the HL-RET-mice (9.7 months; n = 38) was also significantly (p < 0.01) shorter than that in the original RET-mice (10.8 months; n = 20). Since early development of tumors could contribute to shortening of the research period, HL-RET-mice could be a useful model for analysis of melanomagenesis. We then found that the expression level of Mps one binder kinase activator-like-2B (Mobkl2b) in benign tumors was higher than that in malignant melanoma in HL-RET-mice. Expression level of MOBKL2B in malignant melanoma cell lines was also lower than that in non-malignant melanocytic cells in mice and humans, suggesting that MOBKL2B could be a novel marker for malignant melanoma.
Conclusion: We established a novel hairless RET-transgenic mouse line spontaneously developing cutaneous malignant melanomas from benign melanocytic tumors. This mouse model may be useful to find new candidates of melanoma-related molecule.

• 出版日期2012-3