A synthetic biology approach for the transformation of L-alpha-amino acids to the corresponding enantiopure (R)- or (S)-alpha-hydroxy acids

作者：Gourinchas Geoffrey; Busto Eduardo; Killinger Manuela; Richter Nina; Wiltschi Birgit^*; Kroutil Wolfgang

来源：Chemical Communications, 2015, 51(14): 2828-2831.

DOI：10.1039/c4cc08286a

Combinatorial assembly and variation of promoters on a single expression plasmid allowed the balance of the catalytic steps of a three enzyme (L-AAD, HIC, FDH) cascade in E. coli. The designer cell catalyst quantitatively transformed L-amino acids to the corresponding optically pure (R)- and (S)-alpha-hydroxy acids at up to 200 mM substrate concentration.

• 出版日期2015