A carbon nanotube-based electrochemical immunosensor for cardiac troponin T

作者：Gomes Filho S L R; Dias A C M S; Silva M M S; Silva B V M; Dutra R F^*

来源：Microchemical Journal, 2013, 109: 10-15.

DOI：10.1016/j.microc.2012.05.033

• Cardiac troponin T
• Immunosensor
• Carbon nanotubes
• Polyethyleneimine

A nanostructured immunosensor based on carbon nanotubes supported by a conductive polymer film was developed for detection of cardiac Troponin T (cTnT), an important cardiac marker for acute myocardial infarction. Carboxylated carbon nanotubes were covalently bound to the electrode surface via polyethyleneimine. The functionalized nanostructured surface was used to bind anti-cTnT monoclonal antibodies. Stepwise modification of the electrode was characterized by cyclic voltammetry studies. The immunosensor achieved a low limit of detection (0.033 ng mL(-1)) and a linear range between 0.1 and 10 ng mL(-1) cTnT), significant for acute myocardial infarction diagnosis. Good reproducibility and repeatability were obtained by the proposed immunosensor supported by a coefficient of variation of 3.7% and 2.6%, respectively.

• 出版日期2013-7