An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages

作者：Kim Hye Ran; Lee Hyun Su; Lee Kyung Sik; Jung In Duk; Kwon Min Sung; Kim Chang Hyun; Kim Seong Min; Yoon Myung Han; Park Yeong Min; Lee Sang Myeong; Jun Chang Duk^*

来源：Scientific Reports, 2017, 7(1): 8731.

DOI：10.1038/s41598-017-09144-x

Activated macrophages have a greater ability of phagocytosis against pathogens that is mediated by large-scale actin rearrangement. However, molecular machineries that conduct this task have not been fully identified. Here, we demonstrate an unanticipated role of TAGLN2, a 22-kDa actin-binding protein, in Toll-like receptor (TLR)-stimulated phagocytosis. TAGLN2 was greatly induced in macrophages in response to lipopolysaccharide (LPS), a ligand for TLR4, partly via the NF-kappa B pathway. TAGLN2-deficient macrophages (TAGLN2(-/-)) showed defective phagocytic functions of IgM- and IgG-coated sheep red blood cells as well as bacteria. Cell signaling pathways involved in actin rearrangement-PI3 kinase/AKT and Ras-ERK-were also down-regulated in LPS- stimulated TAGLN2-deficient macrophages. Moreover, TAGLN2(-/-) mice showed higher mortality after bacterial infection than wild-type littermates. Thus, our results revealed a novel function of TAGLN2 as a molecular armament required for host defense.

• 出版日期2017-8-18