Subthalamic hGAD65 Gene Therapy and Striatum TH Gene Transfer in a Parkinson's Disease Rat Model

作者：Zheng, Deyu; Jiang, Xiaohua; Zhao, Junpeng^*; Duan, Deyi; Zhao, Huanying; Xu, Qunyuan

来源：NEURAL PLASTICITY, 2013, 2013: 263287.

DOI：10.1155/2013/263287

• AMINO-ACID DECARBOXYLASE
• GTP CYCLOHYDROLASE-I
• TYROSINE-HYDROXYLASE
• GLUTAMATE-DECARBOXYLASE
• L-DOPA
• BEHAVIORAL RECOVERY
• BASAL GANGLIA
• BRAIN-REGIONS
• STEM-CELLS
• EXPRESSION

The aim of the present study is to detect a combination method to utilize gene therapy for the treatment of Parkinson's disease (PD). Here, a PD rat model is used for the in vivo gene therapy of a recombinant adeno-associated virus (AAV2) containing a human glutamic acid decarboxylase 65 (rAAV2-hGAD65) gene delivered to the subthalamic nucleus (STN). This is combined with the ex vivo gene delivery of tyrosine hydroxylase (TH) by fibroblasts injected into the striatum. After the treatment, the rotation behavior was improved with the greatest efficacy in the combination group. The results of immunohistochemistry showed that hGAD65 gene delivery by AAV2 successfully led to phenotypic changes of neurons in STN. And the levels of glutamic acid and GABA in the internal segment of the globus pallidus (GPi) and substantia nigra pars reticulata (SNr) were obviously lower than the control groups. However, hGAD65 gene transfer did not effectively protect surviving dopaminergic neurons in the SNc and VTA. This study suggests that subthalamic hGAD65 gene therapy and combined with TH gene therapy can alleviate symptoms of the PD model rats, independent of the protection the DA neurons from death.

• 出版日期2013
• 单位首都医科大学; 锦州医科大学; 北京市神经外科研究所