A novel 3-Hydroxychromone fluorescence sensor for intracellular Zn2+ and its application in the recognition of prostate cancer cells

作者：Li, Xiang; Li, Jie; Dong, Xiongwei; Gao, Xiang; Zhang, Dan^*; Liu, Changlin^*

来源：Sensors and Actuators B: Chemical , 2017, 245: 129-136.

DOI：10.1016/j.snb.2017.01.170

• Zinc-selective metal chelator
• Biosensors
• Recognition
• Prostate cancer cells
• Bioimaging

A novel fluorescent zinc sensor 3HC-DPA, containing a 3-hydroxychromone (3HC) chromophore and a zinc-selective metal chelator di(2-picolyl)amine (DPA), was prepared and found to chelate Zn2+ in a dimeric form. Due to the deprotonation of 3HC and occupation of the lone electron pairs on the nitrogen atoms of DPA, the complex [Zn-2(3HC-DPA)(2)](2+) emitted sensitively and selectively strong fluorescence in solution. Moreover, cell experiments, including flow cytometry and two-photo excitation fluorescence microscopy, as well as inductively coupled plasma-atomic emission spectrometry (ICP-AES), demonstrated that 3HC-DPA possessed the ability to monitor intracellular free zinc ions in live cells. A promising use was developed for the ability of 3HC-DPA in the recognition of cancerous prostate cells from normal prostate cells, based on the added Zn2+ detection by flow cytometry and cell imaging.

• 出版日期2017-6
• 单位华中师范大学