Modulation of transcriptional activity in brain lower grade glioma by alternative splicing

作者：Li, Jin; Wang, Yang; Meng, Xianglian; Liang, Hong^*

来源：PeerJ, 2018, 6: e4686.

DOI：10.7717/peerj.4686

• Alternative splicing
• Amyloid precursor protein
• EST domain-containing protein Elk-1
• Serine/threonine kinase 16
• Modulator
• Lower grade glioma

Proteins that modify the activity of transcription factors (TFs) are often called modulators and play a vital role in gene transcriptional regulation. Alternative splicing is a critical step of gene processing, and differentially spliced isoforms may have different functions. Alternative splicing can modulate gene function by adding or removing certain protein domains and thereby influence the activity of a protein. The objective of this study is to investigate the role of alternative splicing in modulating the transcriptional regulation in brain lower grade glioma (LGG), especially transcription factor ELK1, which is closely related to various disorders, including Alzheimers disease and Down syndrome. The results showed that changes in the exon inclusion ratio of proteins APP and STK16 are associated with changes in the expression correlation between ELK1 and its targets. In addition, the structural features of the two modulators are strongly associated with the pathological impact of exon inclusion. The results of our analysis suggest that alternatively spliced proteins have different functions in modifying transcription factors and can thereby induce the dysregulation of multiple genes.

• 出版日期2018-5-14
• 单位哈尔滨工程大学