Development of real-time PCR assay using Taq Man probe for detection and quantification of Rosellinia necatrix in plant and soil

作者：Shishido Masahiro^*; Kubota Itsuki; Nakamura Hitoshi

来源：Journal of General Plant Pathology, 2012, 78(2): 115-120.

DOI：10.1007/s10327-012-0366-x

• Rosellinia necatrix
• White root rot
• qPCR
• Tay Man probe
• Japanese pear

We developed real-time PCR assays using TaqMan probes to detect and quantify Rosellinia necatrix, the causal agent of white root rot in many plant species. Two sets of PCR primers and TaqMan probe indicated that their detection limits could be as low as 1 fg of template DNA. Using the real-time PCR assays with the TaqMan probes, we were able to quantify R. necatrix DNA in naturally diseased roots of Japanese pear and in artificially infested soil samples. Although the new assays were inadequate for use with naturally infested soil samples, nested PCR procedures improved the detectability of the new assays.

• 出版日期2012-3