Deciphering the Role of CD1e Protein in Mycobacterial Phosphatidyl-myo-inositol Mannosides (PIM) Processing for Presentation by CD1b to T Lymphocytes

作者：Cala De Paepe Diane; Layre Emilie; Giacometti Gaelle; Garcia Alles Luis F; Mori Lucia; Hanau Daniel; de Libero Gennaro; de la Salle Henri; Puzo Germain; Gilleron Martine^*

来源：Journal of Biological Chemistry, 2012, 287(37): 31494-31502.

DOI：10.1074/jbc.M112.386300

Lipids are important antigens that induce T cell-mediated specific immune responses. They are presented to T lymphocytes by a specific class of MHC-Ilike proteins, termed CD1. The majority of the described CD1-presented mycobacterial antigens are presented by the CD1b isoform. We previously demonstrated that the stimulation of CD1b-restricted T cells by the hexamannosylated phosphatidyl-myo-inositol (PIM6), a family of mycobacterial antigens, requires a prior partial digestion of the antigen oligomannoside moiety by alpha-mannosidase and that CD1e is an accessory protein absolutely required for the generation of the lipid immunogenic form. Here, we show that CD1e behaves as a lipid transfer protein influencing lipid immunoediting and membrane transfer of PIM lipids. CD1e selectively assists the alpha-mannosidase-dependent digestion of PIM6 species according to their degree of acylation. Moreover, CD1e transfers only diacylated PIM from donor to acceptor liposomes and also from membranes to CD1b. This study provides new insight into the molecular mechanisms by which CD1e contributes to lipid immunoediting and CD1-restricted presentation to T cells.

• 出版日期2012-9-7