AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters

作者：Sicard Delphine; Cecioni Samy; Iazykov Maksym; Chevolot Yann; Matthews Susan E; Praly Jean Pierre; Souteyrand Eliane; Imberty Anne; Vidal Sebastien^*; Phaner Goutorbe Magali

来源：Chemical communications, 2011, 47(33): 9483-9485.

DOI：10.1039/c1cc13097h

Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternate 90 degrees orientation of LecA tetramers is proposed to describe the organisation of lectins and glycoclusters in the filaments.

• 出版日期2011