Application of radiation hybrid in gene mapping

作者：He, KL^*; Gu, BW; Zhang, QH; Fu, G; Wu, JS; Han, ZG; Cao, WJ; Zou, J; Mao, M; Liu, JX; Chen, Z; Chen, SJ

来源：Science China Life Sciences, 1998, 41(6): 644-649.

DOI：10.1007/BF02882907

• gene mapping
• radiation hybrid
• hematopoietic regulation

Radiation hybrid (RH) mapping technique was exploited to determine chromosome locations of 26 human novel full length cDNAs recently cloned. All these cDNA clones were isolated from human cord blood CD34+ cells and may be related to regulation of hematopoiesis. 23 genes were successfully mapped to chromosomal positions, while RH analyses were not possible in the remaining 3 cases. RH technique is indeed a powerful tool for mapping never cDNA sequences due to its rapidity, precision, convenience and reproducibility.

• 出版日期1998-12
• 单位上海市闵行区中心医院