科研之友
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摘要
A simple technique for the collection of an extraction solvent lighter than water after dispersive liquidliquid microextraction combined with high-performance liquid chromatography with ultraviolet detection was developed for the determination of four paraben preservatives in aqueous samples. After the extraction procedure, low-density organic solvent together with some little aqueous phase was separated by using a disposable glass Pasteur pipette. Next, the flow of the aqueous phase was stopped by successive dipping the capillary tip of the pipette into anhydrous Na2SO4. The upper organic layer was then removed simply with a microsyringe and injected into the high-performance liquid chromatography system. Experimental parameters that affect the extraction efficiency were investigated and optimized. Under optimal extraction conditions, the extraction recoveries ranged from 25 to 86%. Good linearity with coefficients with the square of correlation coefficients ranging from 0.9984 to 0.9998 was observed in the concentration range of 0.0010.5 mu g/mL. The relative standard deviations ranged from 4.1 to 9.3% (n = 5) for all compounds. The limits of detection ranged from 0.021 to 0.046 ng/mL. The method was successfully applied for the determination of parabens in tap water and fruit juice samples and good recoveries (61108%) were achieved for spiked samples.
- 出版日期2012-10
