Developmental changes in electrophysiological characteristics of human-induced pluripotent stem cell derived cardiomyocytes

作者：Ben Ari Meital; Naor Shulamit; Zeevi Levin Naama; Schick Revital; Ben Jehuda Ronen; Reiter Irina; Raveh Amit; Grijnevitch Inna; Barak Omri; Rosen Michael R; Weissman Amir; Binah Ofer^*

来源：Heart Rhythm, 2016, 13(12): 2379-2387.

DOI：10.1016/j.hrthm.2016.08.045

• iPSC-CMs
• Action potential
• Development
• Beat rate variability

BACKGROUND Previous studies proposed that throughout differentiation of human induced Pluripotent Stem Cell-derived cardiomyocytes (iPSC-CMs), only 3 types of action potentials (APs) exist: nodal-, atrial-, and ventricular-like.
OBJECTIVES To investigate whether there are precisely 3 phenotypes or a continuum exists among them, we tested 2 hypotheses: (1) During culture development a cardiac precursor cell is present that depending on age can evolve into the 3 phenotypes. (2) The predominant pattern is early prevalence of a nodal phenotype, transient appearance of an atrial phenotype, evolution to a ventricular phenotype, and persistence of transitional phenotypes.
METHODS To test these hypotheses, we (1) performed fluorescence-activated cell sorting analysis of nodal, atrial, and ventricular markers; (2) recorded APs from 280 7- to 95-day-old iPSC-CMs; and (3) analyzed AP characteristics.
RESULTS The major findings were as follows: (1) fluorescence activated cell sorting analysis of 30- and 60-day-old cultures showed that an iPSC-CMs population shifts from the nodal to the atrial/ventricular phenotype while including significant transitional populations; (2) the AP population did not consist of 3 phenotypes; (3) culture aging was associated with a shift from nodal to ventricular dominance, with a transient (57-70 days) appearance of the atrial phenotype; and (4) beat rate variability was more prominent in nodal than in ventricular cardiomyocytes, while pacemaker current density increased in older cultures.
CONCLUSION From the onset of development in culture, the iPSCCMs population includes nodal, atrial, and ventricular APs and a broad spectrum of transitional phenotypes. The most readily distinguishable phenotype is atrial, which appears only transiently yet dominates at 57-70 days of evolution.

• 出版日期2016-12