Moderate dependence of reactive oxygen species production on membrane potential in avian muscle mitochondria oxidizing glycerol 3-phosphate

作者：Kikusato Motoi^*; Toyomizu Masaaki

来源：Journal of Physiological Sciences, 2015, 65(6): 555-559.

DOI：10.1007/s12576-015-0395-2

• Mitochondrial glycerol 3-phosphate dehydrogenase
• Mitochondrial membrane potential
• Carbonyl cyanide p-trifluoromethoxyphenyl hydrazine

Mitochondria are a major source of reactive oxygen species production in cells, and the production level is sensitive to the magnitude of the membrane potential (Delta Psi). The present study investigated the level of superoxide production in mitochondria oxidizing glycerol 3-phosphate (GP) and its dependence on Delta Psi in isolated avian muscle mitochondria. The levels of superoxide produced in mitochondria oxidizing GP were lower than those obtained with succinate and were similar to those obtained with NADH-linked substrates (glutamate/malate/pyruvate). The dependence of superoxide production on Delta Psi in mitochondria oxidizing GP was lower than that of mitochondria oxidizing succinate, and a weak dependence of GP-supported superoxide production on Delta Psi was observed in the presence of NADH-linked substrates or succinate. These results suggest that the levels of superoxide generated in response to GP are quantitatively low, but they are unsusceptible to changes in Delta Psi in avian muscle mitochondria.

• 出版日期2015-11