Comparison of HIF1A-AS1 and HIF1A-AS2 in regulating HIF-1α and the osteogenic differentiation of PDLCs under hypoxia

作者：Chen, Dongru; Wu, Liping^*; Liu, Lu; Gong, Qimei; Zheng, Jinxuan; Peng, Caixia; Deng, Jianqing

来源：International Journal of Molecular Medicine, 2017, 40(5): 1529-1536.

DOI：10.3892/ijmm.2017.3138

• hypoxia
• hypoxia-inducible factor-1 alpha
• HIF1A antisense RNA 1
• HIF1A antisense RNA 2
• osteogenic differentiation
• periodontal ligament cells

Hypoxia-inducible factor-1 alpha (HIF-1 alpha) is essential for regulating the osteogenic differentiation of periodontal ligament cells (PDLCs). The regulatory mechanism of HIF-1 alpha transcription is still not clear. Recently, two long non-coding RNAs, HIF1A antisense RNA 1 (HIF1A-AS1) and HIF1A antisense RNA 2 (HIF1A-AS2), were found to regulate HIF-1 alpha mRNA, but the regulatory mechanisms among HIF-1 alpha, HIF1A-AS1 and HIF1A-AS2 have not been well studied. We hypothesized that HIF1A-AS1 and HIF1A-AS2 play important roles in the osteogenic differentiation of PDLCs by regulating HIF-1 alpha. In the present study, we showed that expression levels of HIF1A-AS1, HIF1A-AS2, HIF-1 alpha and osteogenic biomarkers were time-dependent under hypoxia. Even though both HIF1A-AS1 and HIF1A-AS2 were complementary to HIF-1 alpha mRNA, only HIF1A-AS2 showed an inhibitory effect on HIF-1 alpha in PDLCs. Moreover, HIF-1 alpha had positive regulatory effects on HIF1A-AS1 and HIF1A-AS2. HIF-1 alpha promoted the osteogenic differentiation of PDLCs, and HIF1A-AS2 had a negative effect on the osteogenic differentiation of PDLCs. Altogether, the present study revealed the complex relationships among HIF1A-AS1, HIF1A-AS2 and HIF-1 alpha, as well as their roles in regulating the osteogenic differentiation of PDLCs. These findings provide a theoretical basis for promoting periodontal tissue regeneration and repair during orthodontic tooth movement.

• 出版日期2017-11
• 单位中山大学