Development of a Label Free IGE Sensitive Aptasensor Based on Electrochemical Impedance Spectrometry

作者：Kara Pinar^*; Meric Buket; Ozsoz Mehmet

来源：Combinatorial Chemistry & High Throughput Screening, 2010, 13(7): 578-581.

• Aptamer
• IgE
• biosensor
• electrochemical impedance spectrometry (EIS)

A sensitive aptamer based electrochemical biosensor to detect human immunoglobulin E (IgE) is presented in this study. 5' Biotin labeled 45 mer DNA aptamer sequence was immobilized onto streptavidin coated graphite surfaces. Interaction between human IgE and DNA aptamer was monitored by Electrochemical Impedance Spectrometry (EIS) in a 0.48nM detection limit of IgE. EIS analyses are based on electron transfer resistance (R(ct)) in the presence of 5mM [Fe(CN)(6)](3-/4-).

• 出版日期2010-8