Anti-angiogenic effect of siphonaxanthin from green alga, Codium fragile

作者：Ganesan Ponesakki; Matsubara Kiminori; Ohkubo Takeshi; Tanaka Yukihisa; Noda Kenji; Sugawara Tatsuya^*; Hirata Takashi

来源：Phytomedicine, 2010, 17(14): 1140-1144.

DOI：10.1016/j.phymed.2010.05.005

• Angiogenesis
• Siphonaxanthin
• Endothelial cells
• Aortic ring

Since anti-angiogenic therapy has becoming a promising approach in the prevention of cancer and related diseases, the present study was aimed to examine the anti-angiogenic effect of siphonaxanthin from green alga (Codium fragile) in cell culture model systems and ex vivo approaches using human umbilical vein endothelial cells (HUVECs) and rat aortic ring, respectively. Siphonaxanthin significantly suppressed HUVEC proliferation (p < 0.05) at the concentration of 2.5 mu M (50% as compared with control) and above, while the effect on chemotaxis was not significant. Siphonaxanthin exhibited strong inhibitory effect on HUVEC tube formation. It suppressed the formation of tube length by 44% at the concentration of 10 mu M, while no tube formation was observed at 25 mu M, suggesting that it could be due to the suppression of angiogenic mediators. The ex vivo angiogenesis assay exhibited reduced microvessel outgrowth in a dose dependent manner and the reduction was significant at more than 2.5 mu M. Our results imply a new insight on the novel function of siphonaxanthin in preventing angiogenesis related diseases.

• 出版日期2010-12-1