Short-term PTH administration increases dentine apposition and microhardness in mice

作者：Guimaraes Gustavo Narvaes; Camargo Cardoso Guinea Brasil; Naves Lucas Zago; Correr Sobrinho Lourenco; Peres Line Sergio Roberto; Marques Marcelo Rocha^*

来源：Archives of Oral Biology, 2012, 57(10): 1313-1319.

DOI：10.1016/j.archoralbio.2012.03.007

• Parathyroid hormone
• Dentine
• Knoop microhardness
• EDX
• Mice

Objective: The purpose of this study is to investigate the effects of intermittent parathyroid hormone (PTH) administration on the apposition rate and structural features of dentine from mouse incisors.
Methods: Young male A/J Unib mice were treated daily for 6 and 10 days with 40 mu g/kg of hPTH 1-34 or a vehicle. Dentine apposition rates measured by fluorescent labels (tetracycline and calcein) and alkaline phosphatase (ALP) plasma levels were evaluated after 6 days of treatment. Knoop microhardness testing and element content measurements in at.% of calcium (Ca), phosphorus (P), oxygen (0), and magnesium (Mg) in the peritubular and intertubular dentine were performed by Energy Dispersive X-ray (EDX) microanalysis via Scanning Electron Microscopy (SEM) after 10 days of treatment.
Results: Histometric analysis revealed an increase of 5% in the apposition rate of dentine and 25% in the ALP plasma levels in the PTH treated group. In addition, knoop microhardness testing revealed that the animals treated with PTH had a greater microhardness (11%). EDX microanalysis showed that PTH treatment led to increases in P (23%) and Ca (53%) at.% content, as well as the Ca/P ratio (24%) in peritubular dentine. The chemical composition of intertubular dentine did not vary between the groups.
Conclusions: These findings indicate that intermittent administration of hPTH (1-34) increases apposition and mineralization of the dentine during young mice incisor formation.

• 出版日期2012-10