Preparation, characterization, and process performance of composite fibrous adsorbents as cation exchangers for high throughput and high capacity bioseparations

作者：Gavara Poondi Rajesh; Cabrera Rosa; Vennapusa Rami Reddy; Grasselli Mariano; Fernandez Lahore Marcelo^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2012, 903: 14-22.

DOI：10.1016/j.jchromb.2012.06.027

• Composite fibrous adsorbent
• Hydrogel
• Column packing
• Permeability coefficient
• Dynamic binding capacity
• Resolution factor

Fibrous materials are proposed as novel chromatographic supports depicting high throughput and high product capacity. In this work, a composite fiber harboring strong cation-exchange moieties has been investigated. Such materials were characterized by a plethora of physical methods including degree of swelling (DS), scanning electron microscope (SEM), confocal laser scanning microscopy (CLSM), and Fourier transform infrared spectroscopy-attenuated total reflection (FTIR-ATR). The composite showed a high degree of grafting (similar to 30%) and exhibited a high swelling ratio (similar to 300%). Moreover, homogenous grafting and the development of an internal (functional) hydrogel were observed. The fibrous adsorbent was packed utilizing a designed %26quot;double roll%26quot; supported-structure and subsequently tested for packing efficiency and chromatography performance. The mentioned system showed similar packing efficiency of height equivalent to a theoretical plate (HETP) value and higher permeability coefficient (0.92 x 10(-7) cm(2)) than commercial resins. Experimentally determined Peclet number (Pe) values were within the range 60-90, suggesting a close-to-plug-flow condition. Total ionic capacity of the fibrous adsorbent was determined by the transition pH method. A capacity of 6.5 mequiv./g was obtained. Moreover, a high dynamic binding capacity for lysozyme was found to be 283 mg/g. On the other hand, a bed of randomly packed fiber also demonstrated high-resolution ability when a mixture of model protein was utilized to that end. Resolution was maintained at high flow rates (up to 900 cm/h) and utilizing shorter gradient development routines. Direct sequestration of a model protein (lysozyme) was also possible from an artificial mixture containing 1.5% yeast homogenate. Summarizing, the composite fibrous adsorbents exhibited superior performance during early protein capture and intermediate-resolution applications.

• 出版日期2012-8-15