摘要
In this work, a dicyanoisophorone-based fluorophore, which showed an attractive near-infrared fluorescence as well as a favorable large stokes shift, was used to develop a novel probe (NIR-Probe 1) for effective detection of biothiols. It displayed distinct color changes and a turn-on NIR fluorescence response with a high off/on ratio toward biothiols. We demonstrated that NIR-Probe 1 exhibited fast sensing speed, high selectivity for biothiols and possessed the function of detecting low concentration of biothiols with impressive limit of detection: 12.1 nM for Cys, 14.5 nM for Hcy and 11.9 nM for GSH. Moreover, the utility of NIR-Probe 1 as a bioanalytical molecular tool was demonstrated by fluorescence imaging of biothiols in living cells.
- 出版日期2017-9
- 单位华东理工大学; 生物反应器工程国家重点实验室; 上海市质量监督检验技术研究院