科研之友
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摘要
Ultra high sensitive and simple detection of DNA is crucial to early diagnosis of diseases and biodefense application. Herein, we report an amplified chemiluminescence (CL) biosensing platform for ultralow concentration DNA detection. It is based on the distinguishing ability of positively charged gold nanoparticles ((+)AuNPs) between ss-DNA and ds-DNA. (+)AuNPs' effect on luminol CL reaction is used to amplify the response signal of target DNA. Moreover, the three key experimental procedures (DNA hybridization, ss-DNA action on (+)AuNPs and CL detection) in the proposed strategy are independent so that the three experiments can occur in the own optimal condition, respectively. (+)AuNPs' direct effect produced by different structure DNAs (ss-DNA and ds-DNA) can make CL signal to be significantly amplified. The proposed biosensing has high sensitivity for detecting target DNA. The detection limit of target DNA was 0.62 fM, which was lower than that of the reported DNA assays based on other signal amplification. Also, the present method is more convenient. Such direct signal amplification from nanometer interface effect will provide a promising alternative avenue for the construction of high sensitive CL sensing platform. It is especially noteworthy that the action mechanisms of AuNPs and DNA can be clarified by this research, which will be important theoretical support for the design of DNA biosensors.
- 出版日期2018-5-1
- 单位西安科技大学; 福建工程学院
