Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1

作者：Wang Pingzhang^*; Xiong Ying; Ma Chuan; Shi Taiping; Ma Dalong

来源：BMB Reports, 2010, 43(11): 738-743.

DOI：10.3858/BMBRep.2010.43.11.738

• AP-1
• Cloning
• JNK2
• MAPK9
• Transcript variant

The c-Jun NH(2)-terminal kinase (JNK) signaling pathway participates in many physiological functions. In the current study we reported the cloning and characterization of five novel JNK2 transcript variants, which were designated as INK2 alpha 3, JNK2 alpha 4, JNK2 beta 3, JNK2 gamma 1 and JNK2 gamma 2, respectively. Among them, JNK2 alpha 4 and JNK2 gamma 2 are potential non-coding RNA because they contain pre-mature stop codons. Both JNK2 alpha 3 and JNK2 beta 3 contain an intact kinase domain, and both encode a protein product of 46 kDa, the same as those of JNK2 alpha 1 and JNK2 beta 1. JNK2 gamma 1 contains a disrupted kinase domain and it showed a disable function. When over-expressed in mammalian cells, JNK2a3 showed higher activity on AP-1 than that of JNK2 beta 3 and JNK2 gamma 1. Furthermore, JNK2 alpha 3 and JNK2 beta 3 showed different levels of substrate phosphorylation, although they both could promote the proliferation of 293T cells. Our results further demonstrate that JNK2 isoforms preferentially target different substrates and may regulate the expression of various target genes. [BMB reports 2010; 43(11): 738-743]

• 出版日期2010-11-30
• 单位北京大学