Waxy allelic diversity in common millet (Panicum miliaceum L.) in China

作者：Wang, Ruiyun^*; Wang, Haigang; Liu, Xiaohuan; Ji, Xu; Chen, Ling; Lu, Ping; Liu, Minxuan; Teng, Bin; Qiao, Zhijun^*

来源：Crop Journal, 2018, 6(4): 377-385.

DOI：10.1016/j.cj.2018.02.004

• Common millet
• Waxy gene
• SNP
• Starch

A set of 132 accessions of common millet, Panicum miliaceum L., from 12 provinces of China were assessed for endosperm starch type (waxy or non-waxy) using I-2-HI staining, amylose and amylopectin contents using the dual-wavelength coloilmetric method, and genotype of Waxy genes conditioning amylose content by gene sequencing. Endosperm starch content varied from 57.69% to 74.70%, while the amylose and amylopectin contents of the starch ranged from 0 to 23.29% and from 41.99% to 70.24%, respectively. Sequencing two Wx genes, including Wx-L (intron 5-exon 7 and intron 8-9) and Wx-S (exon 9-intron 10) revealed several polymorphisms (S-0, S-15, L-C, L-F, L-Y). Marker M5-R11 linking to the Wx-S gene may be used to discriminate waxy common millet accessions from non-waxy ones. Among the 132 accessions, 68 with the S-15 allele had waxy endosperm starch with the amylose content range 0-2.58% and 64 accessions with the So allele had non-waxy endosperm starch with amylose content range 3.94%-23.29%. Five genotypes including S-15/L-F (45% of the accessions), S-0/L-F (25%), S-0/L-Y (12%), S-0/L-C (11%), and S-15/L-Y (7%) were identified. Six new SNPs were detected at the Wx-L locus. These results will facilitate common millet breeding, especially of cultivars free of amylose.

• 出版日期2018-8
• 单位中国农业科学院; 山西农业大学; 安徽省农业科学院