Understanding fatty acid synthesis in developing maize embryos using metabolic flux analysis

作者：Alonso Ana Paula^*; Dale Val L; Shachar Hill Yair

来源：Metabolic Engineering, 2010, 12(5): 488-497.

DOI：10.1016/j.ymben.2010.04.002

• Zea mays
• Metabolic flux analysis
• Carbon conversion efficiency
• Maize embryo
• Fatty acid synthesis
• NADP-dependent malic enzyme

The efficiency with which developing maize embryos convert substrates into seed storage reserves was determined to be 5771%, by incubating developing maize embryos with uniformly labeled C-14 substrates and measuring their conversion to CO2 and biomass products. To map the pattern of metabolic fluxes underlying this efficiency, maize embryos were labeled to isotopic steady state using a combination of labeled C-13-substrates. Intermediary metabolic fluxes were estimated by computer-aided modeling of the central metabolic network using the labeling data collected by NMR and GC-MS and the biomass composition. The resultant flux map reveals that even though 36% of the entering carbon goes through the oxidative pentose-phosphate pathway, this does not fully meet the NADPH demands for fatty acid synthesis. Metabolic flux analysis and enzyme activities highlight the importance of plastidic NADP-dependent malic enzyme, which provides one-third of the carbon and NADPH required for fatty acid synthesis in developing maize embryos. Published by Elsevier Inc.

• 出版日期2010-9