Novel hydroxyethylcellulose-graft-poly acrylamide copolymer for separation of double-stranded DNA fragments by CE

作者：Yang Runmiao; Wang Yanmei^*; Zhou Dan

来源：Electrophoresis, 2007, 28(18): 3223-3231.

DOI：10.1002/elps.200600818

• atom transfer radical graft polymerization
• double-stranded DNA
• graft copolymer

A novel separation medium, hydroxyethylcellulose-graft-polyacrylamide (HEC-g-PAM) synthesized by atom transfer radical polymerization (ATRP), used for dsDNA separation by CE is presented. The separation performance of HEC-g-PAM, which has the same graft density and different graft length, has been investigated in Tris-boric acid-EDTA (TBE) buffer solvent mixtures. The temperature-dependent rheological behavior of HEC-g-PAM was also studied by steady-shear rheometry. The results showed that dsDNA fragments between 72 and 1353 bp was achieved with a 30 cm effective capillary length at 150 V/cm using this type of graft copolymer as a separation medium in bare fused-silica capillaries, and separation improvement is obtained in HEC-g-PAM compared with HEC and poly(dimiethylacrylamide) (PDMA).

• 出版日期2007-9
• 单位中国科学技术大学