Porcine extracellular matrix scaffolds in reconstructive urology: An ex vivo comparative study of their biomechanical properties

作者：Davis N F; Callanan A; McGuire B B; Mooney R; Flood H D; McGloughlin T M^*

来源：Journal of the Mechanical Behavior of Biomedical Materials, 2011, 4(3): 375-382.

DOI：10.1016/j.jmbbm.2010.11.005

• Tissue engineering
• Reconstructive urology
• Urinary bladder matrix
• Bladder capacity
• Bladder compliance

Functional reconstruction of the human urinary bladder has been attempted by replacing defective bladder tissue with tissue-engineered xenogenic extracellular matrix (ECM) scaffolds. However, experimental studies that demonstrate the effects of implanted ECMs on important biomechanical properties such as total bladder capacity (TBC) and compliance (C) are lacking. In the current study, the effects of ECM scaffold surface area (SA) on TBC and C was assessed, ex vivo, in an ovine model (n = 5). TBC and C were measured at pressures (P) of 5, 10, 15 and 20 mm Hg prior to performing a 3 x 3 cm (9 cm(2)) partial cystectomy defect. Equal-sized 3 x 3 cm (9 cm2) and larger 6 x 6 cm (36 cm2) urinary bladder matrix (UBM) scaffolds of porcine origin replaced the 3 x 3 cm cystectomy defect, and TBC and C were re-recorded for comparative analysis. The results showed that TBC decreased by 39.6%+/- 0.005% (122.9 ml +/- 15 ml, p < 0.05) and C by 38.9% +/- 0.51%, (Delta P = 0-5 mm Hg, p < 0.05) in ovine bladders reconstructed with 3 x 3 cm UBM scaffolds compared to their native values. It was also found that TBC increased by 25.6 +/- 0.64% (64.2 ml +/- 8.8 ml, p > 0.05) and C by 24.5 +/- 0.43% (Delta P = 0-5 mm Hg, p > 0.05) in the 6 x 6 cm UBM scaffold group compared to the 3 x 3 cm UBM scaffold group; however, these values were not statistically significant. The present work demonstrates that a fourfold increase in ECM scaffold SA relative to its intended defect does not lead to a significant improvement in TBC and C values.

• 出版日期2011-4