Streptomyces swartbergensis sp nov., a novel tyrosinase and antibiotic producing actinobacterium

作者：le Roes-Hill Marilize^*; Prins Alaric; Meyers Paul R.

来源：Antonie van Leeuwenhoek, 2018, 111(4): 589-600.

DOI：10.1007/s10482-017-0979-3

• Streptomyces
• Polyphasic taxonomy
• Tyrosinase
• Antimicrobial

As part of an antibiotic screening program, an actinobacterium, strain HMC13(T), was isolated from soil collected from the banks of the Gamka River, Western Cape Province, South Africa. The isolate was found to produce branched mycelia that differentiated into spiral spore chains with spiny spores. 16S rRNA gene sequence analysis showed the strain to be closely related to Streptomyces caelestis NRRL 2418(T) (99.72%) and Streptomyces azureus NBRC 12744(T) (99.51%). Chemotaxonomic analyses confirmed the classification of the strain as a member of the genus Streptomyces: LL-DAP in the peptidoglycan, no diagnostic sugars in the whole cell sugar pattern, dominant menaquinones including MK9(H-8), MK9(H-6), and the polar lipids detected included phosphatidylethanolamine. The fatty acid profile revealed the presence of mostly branched, saturated fatty acids: iso-C-15:0 (14.4%), anteiso-C-15:0 (21.1%), iso-C-16:0 (16.8%), C-16:1 omega 7c/2-OH iso-C-15:0 (5.8%), C-16:0 (6.2%), iso-C-17:1 omega 9c (5.8%), iso-C-17:0 (5.9%), and anteiso-C-17:0 (9.6%). Strain HMC13(T) is a tyrosinase producer and exhibits very strong antibiosis against Mycobacterium aurum A+ and Staphylococcus aureus subsp. aureus ATCC 33591 (methicillin resistant), while only weak activity was observed against Bacillus cereus ATCC 10876, Enterococcus faecium VanA (vancomycin resistant), Enterococcus faecalis ATCC 51299 (vancomycin resistant) and Candida tropicalis ATCC 750(T). Strain HMC13(T) (= LMG 28849(T) = NRRL B-65294(T)) is proposed as the type strain of a new species, to be named Streptomyces swartbergensis sp. nov.

• 出版日期2018-4