Anti-inflammatory effect of methanolic extract of Conyzacanadensis in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophage cells

作者：Song, Jia-Le; Yi, Ruo-Kun; Gao, Yang^*

来源：Pakistan Journal of Pharmaceutical Sciences, 2016, 29(3): 935-940.

• Conyzacanadensis
• anti-inflammation
• RAW264.7 cells

The aim of this study was to investigate the potential anti-inflammatory effect of Conyzacanadeusis methanol extract (CME) using a cell model of RAW264.7 murine macrophage cell stimulated with lipopolysaccharide (LPS)(1 mu g/ml). Co-treatment with different concentrations (10, 50 and 100 mu g/ml) of CME was concentration-dependently reduced the LPS-induced generation of prostaglandin E2 (PGE2), nitric oxide (NO) tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1 beta and IL-6. In addition, CME also reduced the mRNA expressions of cyclooxygenase-2 (COX-2), inducible nitric oxide (iNOS), TNF-alpha, IL-1 beta and IL-6 in LPS-stimulated RAW264.7 cells. These results suggested that CME showed an anti-inflammatory activity through reduced the mRNA expression of COX-2, iNOS, TNF-alpha, IL-1 beta and IL-6 and also decreased the productions of PGE2, NO, TNF-alpha, IL-1 beta and IL-6in LPS-stimulated RAW264.7 cells.

• 出版日期2016-5
• 单位扬州大学; 桂林医学院