Are Basophil Activation and Sulphidoleukotriene Determination Useful Tests for Monitoring Patients With Peach Allergy Receiving Sublingual Immunotherapy With a Pru p 3-Enriched Peach Extract?

作者：Garrido Fernandez S^*; Garcia B E; Sanz M L; Echechipia S; Lizaso M T; Tabar A L

来源：Journal of Investigational Allergology and Clinical Immunology, 2014, 24(2): 106-113.

• Basophil
• CAST
• Food immunotherapy
• Lipid transfer protein
• Sublingual immunotherapy
• Peach immunotherapy
• LTP

Introduction: Treatment of food allergy essentially consists of food avoidance, but immunotherapy with food is emerging as a new therapeutic option. %26lt;br%26gt;Objective:To evaluate clinical improvement and immunological changes in patients with peach allergy following sublingual immunotherapy (SLIT) with a Pru p3 quantified peach extract. %26lt;br%26gt;Methods: A randomized, double-blind, placebo-controlled clinical trial with peach SLIT was conducted. We assessed clinical efficacy after 6 months of treatment by means of double-blind, placebo-controlled oral challenges with peach and also evaluated immunological changes (basophil activation test [BAT] and determination of sulphidoleukotriene production) following stimulation with peach peel and pulp, rPru p3, rMal d1, and rMal d4 stimulation. We also measured specific IgE and IgG(4) to Pru p 3. %26lt;br%26gt;Results: After 6 months of SLIT (T6), the active group showed a 3-fold improvement in tolerance to Pru p3 and a significant increase in IgE to rPru p3 and in sLT production following stimulation with peach peel and rPru p3. There was also a significant increase in BAT results after stimulation with rPru p3 at 1 month of SLIT (T1). Statistically significant between-group differences were only observed for BAT with peach peel and pulp at T1 and T6 and for BAT with rPru p3 at T6. No changes were observed in BAT with rMal d1 or rMal d4 or in IgG4 levels to nPru p 3. %26lt;br%26gt;Conclusions: SLIT with a Pru p 3 quantified peach extract is clinically effective and leads to an increase in basophil activation and sulphidoleukotriene production following stimulation with rPru p3 and peach peel in the first months of treatment.

• 出版日期2014