Apoptosis-Inducing Factor Is Involved in Gentamicin-Induced Vestibular Hair Cell Death

作者：Zhang Daogong; Fan Zhaomin; Han Yuechen; Bai Xiaohui; Liu Wenwen; Lu Sumei; Wang Mingming; Xu Lei; Luo Jianfen; Li Jianfeng; Wang Haibo^*

来源：ORL: Journal for Oto-Rhino-Laryngology and Its Related Specialties , 2012, 74(1): 1-5.

DOI：10.1159/000333251

• Apoptosis-inducing factor
• Vestibular sensory cell
• Apoptosis

Aim: Vestibular hair cell loss in response to different stimuli may be attributable to the occurrence of apoptosis, in which apoptosis-inducing factor (AIF) is an important regulator mediating apoptotic process independent of caspases. This study was designed to investigate the possible involvement of AIF in gentamicin (GM)-induced vestibular hair cell death. Methods: Vestibular organs from postnatal day 3 or 4 rats were maintained in tissue culture and were exposed to 2 mg/ml GM for up to 72 h. Vestibular hair cell viability was quantified by MTT assay. Apoptosis was determined by flow cytometry. AIF activation was examined by RT-PCR. The expressions of the mitochondrial protein and cytoplasm protein of AIF were detected by Western blot. Results: GM could significantly inhibit the cell viability of vestibular hair cells in a dose-and time-dependent manner. The number of apoptotic cells treated with GM was higher than that of cells not treated with GM. RT-PCR showed upregulation of AIF mRNA under GM. Western blot showed that AIF from mitochondria was decreased, whereas AIF from cytoplasm was increased after GM exposure. Conclusions: AIF participates in GM-induced apoptosis of vestibular hair cells.

• 出版日期2012-2
• 单位山东大学