科研之友
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摘要
A response surface analysis with a face centered central composite design was implemented to study the effect of maize (Zea mays L) starch concentration (3.0-7.0% w/v) and leaching temperature (LT, 70 90 degrees C) on aqueous leaching of amylose (AM) as a way to optimize the conditions for obtaining the highest yield of long chain AM [number average degree of polymerization (DPn) ranging between 860 and 930] and highest purity. Second order empirical models were fitted via the least squares approach. Negligible terms were removed using backwards model reduction. Negligible lack of fit terms were obtained for the responses total leached carbohydrate and DPn. The optimization was complemented with a desirability test using the purity of the extracts. As targets for the optimization, maximum leachate yields, DPn approximate to 900, and purity > 95% were set. The reported contour plots and prediction profilers can be used for tailor made production of leachates. Temperature had the most significant main effect as yields and DPn increased with temperature at the expense of purity. Purity was highly compromised when treatments were above 85 degrees C. This was reflected in the high DPn values (> 1500) which suggested the presence of amylopectin material. When using 3.0% (w/v) maize starch suspension at an LT of 81 degrees C, the largest yield (15.0% of starch) of high DPn AM chains (DPn 900) and less than 33% of non-AM material were obtained.
- 出版日期2017-2
