Application of silver nanoparticles decorated with beta-cyclodextrin in determination of 6-mercaptopurine by surface-enhanced Raman spectroscopy

作者：Yang Lei; Chen Yanhua; Li Hongli; Luo Lan; Zhao Ying; Zhang Hanqi; Tian Yuan^*

来源：Analytical Methods, 2015, 7(16): 6520-6527.

DOI：10.1039/c5ay01212k

Although classical silver nanospheres were widely used as probes in surface-enhanced Raman spectroscopy (SERS), they lacked long-term stability. In the present work, silver nanoparticles decorated with beta-cyclodextrin (beta-CD-AgNPs) were used as probes and the stability was improved. The adsorption characteristics of 6-mercaptopurine (6-MP) on classical silver nanospheres and beta-CD-AgNPs were studied cautiously using SERS, and chemical information about the molecular structure was obtained. The binding of the 6-MP molecule to the surface of AgNPs is based on the stable Ag-S covalent bond. The electrostatic adsorption between the protonated N-7 atom or N-1 atom and the silver surface results in a notable Raman enhancement. The intensity of the Raman band of 6-MP at 866 cm(-1) was used to determine 6-MP. Experimental conditions, such as the volume of CD-AgNP solution, incubation time, pH value and concentration of sodium chloride, were examined and optimized. Compared with classical silver nanospheres, when beta-CD-Ag nanoparticles were used, the sensitivity was higher, the analytical time was shorter, and the linear range was wider. A good linearity (r = 0.996) in the range of (0.040-2.0) x 10(-7) mol L-1 was obtained for determining 6-MP. The limit of detection was 0.024 x 10(-7) mol L-1 and the limit of quantification was 0.080 x 10(-7) mol L-1. This method was successfully applied to the determination of 6-MP in real samples and the results were satisfactory.

• 出版日期2015
• 单位吉林大学