Asymmetric dimethylarginine impairs fibrinolytic activity in human umbilical vein endothelial cells via p38 MAPK and NF-kappa B pathways

作者：Zhang Qin; Chen NaPing; Qiu WenBing; Xu XinWu; Wang DongMing; Tsao Philip S^*; Jin Hong

来源：Thrombosis Research, 2011, 128(1): 42-46.

DOI：10.1016/j.thromres.2011.02.013

• Asymmetric dimethylarginine (ADMA)
• Tissue plasminogen activator (tPA)
• plasminogen activator inhibitor-1 (PAI-1)
• Mitogen activated protein kinase (MAPK)
• Nuclear factor-kappaB (NF-kappa B)

Introduction: Asymmetric dimethylarginine (ADMA) is a potent endogenous inhibitor of nitric oxide (NO) synthase. An increased synthesis and/or a reduced catabolism of ADMA might contribute to the onset and progression of thrombosis. The present study was designed to evaluate the effect of ADMA on fibrinolytic factors in endothelial cells, and to investigate the cellular mechanisms.
Materials and Methods: Human umbilical vein endothelial cells (HUVECs) were treated with different concentrations of ADMA for various periods; Then HUVECs were preincubated with NO precursor (L-arginine), MAPK inhibitors, or NF-kappa B inhibitor (PDTC) before ADMA treatment to repeat the experiment. Protein levels of tissue plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1), and NF-kappa B activity were measured by ELISA; mRNA levels of tPA and PAI-1 were assayed by qRT-PCR; The activation of MAPK was characterized by western blot analysis.
Results: (1) ADMA decreased tPA antigen levels in time- and concentration-dependent manners, with the maximum effect of 30 mu mol/L ADMA for 48 h (control 109.01 +/- 4.15 ng/ml vs ADMA 86.76 +/- 5.95 ng/ml, p<0.01); (2) 30 mu mol/L ADMA elevated antigen levels of PAI-1 in a time-dependent manner, with the maximum effect of 30 mu mol/L ADMA for 48 h (control 2721.12 +/- 278.02 ng/ml vs ADMA 3435.78 +/- 22.33 ng/ml, p<0.05); (3) ADMA reduced tPA mRNA levels and increased PAI-1 mRNA levels; (4) L-arginine, SB203580 (p38 MAPK inhibitor) and PDTC attenuated the effects of ADMA on tPA and PAI-1 significantly. (5) ADMA induced a rapid phosphorylation of p38 MAPK, and stimulated NF-kappa B activity greatly.
Conclusions: ADMA may accelerate thrombosis development by impairing fibrinolytic activity in vascular via inhibiting nitric oxide production and then activating its downstream p38 MAPK and NF-kappa B pathways.

• 出版日期2011-7
• 单位汕头大学