科研之友
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摘要
Although biochars show promise for soil remediation, their negative impacts have not been systematically assessed. This study investigated the effects of corn cob biochars on urease-mediated urea hydrolysis and attempted to determine the mechanisms underlying those effects. The results showed that biochars inhibited urease activity (indicated by the NH4+ generation). Released heavy metals and polyaromatic hydrocarbons explained the 20% inhibition of urease activity in biochar supernatants (200 degrees C) but could not explain the 70% inhibition in particle systems (400 degrees C). The levels of protein carbonyl and nitrotyrosine, common oxidative damagers of urease, were high in particle systems but very low in biochar supernatants. The electron paramagnetic resonance signal intensity reached its highest level in the 400 degrees C biochar and decreased in the 500 degrees C biochar, possibly due to the decomposition of organic molecules. The observed inhibition of urease activity may be the result of oxidative reactions with free radicals on the biochar surface or oxidative reactions with reactive oxygen species promoted by free radicals. We suggest that these potential hazards be evaluated further to gauge the relevance of these findings to field conditions and to assist in the development of safe and sustainable application schemes for biochars.
- 出版日期2018-11-6
- 单位昆明理工大学
