Disparate Hypervariable Region-1 of Mitochondrial DNA Did Not Induce Skin Allograft Rejection in Cloned Porcine Models

作者：Kwak H H; Park K M; Nam H S; Park S M; Woo H M^*

来源：Transplantation Proceedings, 2013, 45(5): 1787-1791.

DOI：10.1016/j.transproceed.2013.01.023

Introduction. Alloantigen recognition in skin transplantation is the bane for surgeons. Several studies have mainly focused on the immunogenicity of major histocompatibility (MHC) antigens and H-Y minor histocompatibility antigens. However, the roles of the mitochondrial DNA (mtDNA) encorded miHA have not been identified. Therefore, we sought to address the antigenicity of the hypervariable region 1 (HV-1) of mtDNA in skin transplantation using cloned pig models. %26lt;br%26gt;Methods. Swine leukocyte antigen and HV-1 of mtDNA were analyzed using sequencing methods. Skin transplantation was performed between MHC-matched, mtDNA-mismatched cloned miniature pigs. Full-thickness skin was grafted between cloned pigs without any immunosuppressants. The grafted tissues were observed for 3 months and evaluated histologically. %26lt;br%26gt;Results. The cloned pigs shared identical MHC but mtDNA mismatched at 9 positions. Skin grafts between the cloned pigs were accepted and hair growth maintained, whereas MHC-mismatched grafts showed acute rejection within 7 days after transplantation and were replaced by hairless scar tissue. %26lt;br%26gt;Conclusions. HV-1 disparate skin grafts were not recognized as alloantigenic by MHC-matched cloned pigs.

• 出版日期2013-6