An improved system to establish highly embryogenic haploid cell and protoplast cultures from pollen calluses of maize (Zea mays L.)

作者：He Guangyuan^*; Zhang Jinrui; Li Kexiu; Xiong Zhiyong; Chen Mingjie; Chang Junli; Wang Yuesheng; Yang Guangxiao; Barnabas Beata

来源：Plant Cell, Tissue and Organ Culture, 2006, 86(1): 15-25.

DOI：10.1007/s11240-006-9091-5

• anther culture
• plant regeneration
• protoplast culture
• type II callus
• Zea mays

Regenerable, embryogenic haploid cell suspensions were initiated and established from type II pollen calluses of two selected Chinese maize genotypes (No 592 Y and 592.A2 LY). The induction frequency of friable, embryogenic callus (type II) was highly dependent on three factors: genotype, medium, cold pretreatment, and on their interactions. Repeated callus and cell selection during the culture procedure led to stable haploid suspensions consisting of fine clusters each containing 20-50 cells. The selected cell lines were able to maintain their morphogenic ability during long-term subculture (2 years). Protoplasts were successfully isolated from subcultured, friable, embryogenic pollen calluses and cultured on N6BM and N6K media using a feeder layer, obtained from 2-day-old suspension culture. Healthy plants were regenerated from protoplast-derived calluses.

• 出版日期2006-7
• 单位华中科技大学