Application of Modified Mesoporous Silica Materials for Analysis of Small Molecules by MALDI-TOF-MS

作者：Dong Mei Hua; Sun Shi Mei; Jin Biao^*

来源：Chinese Journal of Analytical Chemistry, 2014, 42(9): 1235-1239.

DOI：10.1016/S1872-2040(14)60763-5

• Mesoporous silica
• Small organic molecules
• Matrix-assisted laser desorption/ionization time of flight mass
• spectrometry

Mesoporous silica SBA-15 with a small organic molecular (1,8-naphthalimide) was synthesized as the matrix for matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) analysis. A modified SBA-15 matrix, SBA-15-NA (1,8-naphthalimide) was synthesized by covalently attaching SBA-15 to an 1,8-naphthalimide formed by 1,8-naphthalic anhydride and 3-(trimethoxysilyl) propylamine. The mass spectra obtained with SBA-15-NA exhibited less background interference ions, higher desorption/ionization efficiency, higher peak intensity. The matrix were initially prepared in methanol and mixed with analyte at a 1:1 volume ratio in vial. The solution of mixture was at a 10: 1 concentration ratio of matrix/analyte on a stainless steel target and allowed to air dry. The method was used to analyze various types of low molecular weight (less than 500) chemical compounds such as monosaccharides, amino acids, phytohormones, drugs. Under the optimum conditions, a low detection limit of 1 x 10(-9) g L-1 (Leucine, S/N = 5) and a good reproducibility of <= 30% were obtained. The modified mesoporous silica materials were employed in the urinary study for the direct detection of metabolites.

• 出版日期2014-9
• 单位延边大学