A new derivative for oxosteroid analysis by mass spectrometry

作者：Rigdova K; Wang Y; Ward M; Griffiths W J^*

来源：Biochemical and Biophysical Research Communications, 2014, 446(3): 762-767.

DOI：10.1016/j.bbrc.2014.01.190

• Steroids
• Tandem mass spectrometry
• Derivatisation
• Steroid glucuronides
• Urine

Here we report a new method for oxosteroid identification utilizing %26quot;tandem mass tag hydrazine%26quot; (TMTH) carbonyl-reactive derivatisation reagent. TMTH is a reagent with a chargeable tertiary amino group attached through a linker to a carbonyl-reactive hydrazine group. Thirty oxosteroids were analysed after derivatisation with TMTH by electrospray ionization mass spectrometry (ESI-MS) and were found to give high ion-currents compared to underivatised molecules. ESI-tandem mass spectrometry (MS/MS) analysis of the derivatives yielded characteristic fragmentation patterns with specific mass reporter ions derived from the TMT group. A shotgun ESI-MS method incorporating TMTH derivatisation was applied to a urine sample.

• 出版日期2014-4-11