A routine method for the quantification of the novel antimycotic drug posaconazole in plasma using liquid chromatography-tandem mass spectrometry

作者：Vogeser Michael^*; Rieger Christina; Ostermann Helmut; Spoehrer Ute

来源：Clinical Chemistry and Laboratory Medicine, 2009, 47(5): 579-584.

DOI：10.1515/CCLM.2009.129

• antimycotic drugs
• fungal infections
• liquid chromatography-tandem mass spectrometry (LC-MS/MS)
• posaconazole

Background: Posaconazole is now widely used for prophylaxis of invasive fungal infections in immuno-compromised patients. The pharmacokinetic properties of the drug argue for therapeutic monitoring, but so far described analytical methods have shortcomings with respect to application in a routine setting. The aim of our work was to develop an analytical method suitable for routine use.
Methods: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used. For sample preparation, protein precipitation followed by on-line solid phase extraction was used. SCH 56984, a posaconazole related compound provided by the manufacturer of posaconazole, was used as internal standard.
Results: The method was characterized by short hands-on time and an analytical run time of four minutes. Validation data demonstrated acceptable analytical performance.
Conclusions: LC-MS/MS with on-line solid phase extraction for sample preparation allows the implementation of a convenient and reliable method for pharmacokinetic monitoring of posaconazole. Clin Chem Lab Med 2009;47;579-84.

• 出版日期2009-5