Determination of essential oil contents and micropropagation of Gaultheria fragrantissima, an endangered woody aromatic plant of India

作者：Bantawa P; Da Silva J A T; Ghosh S K; Mondal T K^*

来源：Journal of Horticultural Science & Biotechnology, 2011, 86(5): 479-485.

Leaves of mature of Gaultheria fragrantissima Wall. plants (Indian wintergreen) were collected from various locations in the Eastern Himalayan region on the Indo-China border and were analysed by steam distillation and gas chromatography to identify an elite line that contained 1.79% (v/v) essential oils, 98% of which was methyl salicylate. Subsequently, a highly reproducible micropropagation protocol using adult shoot tips from this elite genotype was developed in order to conserve this highly-valued, endangered, woody oil-bearing aromatic shrub in India. Among several plant growth regulator (PGR) combinations tested for in vitro multiplication, up to 35 shoots per explant could be induced within 14 weeks of culture on woody plant medium (WPM) fortified only with 0.22 mg l(-1) thidiazuron (TDZ). These shoots could elongate on WPM containing 1.0 mg l(-1) kinetin. Rooted plantlets were acclimatised ex vivo, with 70% success. Random amplified polymorphic DNA (RAPD) analysis indicated the genetic uniformity of both the micropropagated plantlets and the donor plants. This is the first report on in vitro micropropagation of G. fragrantissima.

• 出版日期2011-9