Droplet-based Blood Hepatitis B Virus DNA Extraction in a Capillary

作者：Liang Guang Tie; Wang Qiu Ping; Wang Wei; Liu Da Yu^*; Zhou Xiao Mian

来源：Chinese Journal of Analytical Chemistry, 2011, 39(5): 670-674.

DOI：10.3724/SP.J.1096.2011.00670

• Micro-total nucleic acid analysis system
• Deoxyribonucleic acid extraction
• Droplet
• Capillary
• Hepatitis B virus

A novel in-capillary droplet-based DNA extraction method was developed, using the principle of water-in-oil droplet formation in the highly hydrophobic polytetrafluoroethylene capillary. By sequentially introducing droplets containing sample or reagents, all the steps related to blood hepatitis B virus (HBV) DNA extraction, including sample injection, DNA binding, magnetic beads rinsing and DNA elution were continuously implemented in the capillary. Experimental results showed that proteinase K lysis and elevated elution temperature facilitated higher extraction efficiency. With optimized conditions, an extraction was completed in 25 min with microliter level sample/reagent consumption. The droplet-based method achieved an extraction efficiency of similar to 60% that is comparable to that of a commercial method. However, the RSD of extraction efficiency was relatively higher. In summary, the droplet-based DNA extraction developed is simple, fast and low-cost. It has the potential to be integrated into a micro-total nucleic acid analysis system.

• 出版日期2011-5
• 单位广州医科大学