A new method for analyzing gene expression based on frequency analysis of RT-PCR products obtained with degenerate primers

作者：Kiselev Konstantin V^*; Dubrovina Alexandra S

来源：Acta Physiologiae Plantarum, 2010, 32(3): 495-502.

DOI：10.1007/s11738-009-0426-9

• Degenerate primers
• Gene expression analysis
• Expression profiles

This paper presents a new method of gene expression analysis: frequency analysis of RT-PCR products obtained with degenerate primers (FAPP). The main advantage of the new approach compared to the present methods of gene expression analysis is that it is applicable to non-model plant objects whose gene sequences are unknown. The advantages and disadvantages of FAPP are described in detail using data on calcium-dependent protein kinase, stilbene synthase, and phenylalanine ammonia-lyase gene expression in two cell cultures of Vitis amurensis. We compared the expression profiles obtained by FAPP to those obtained by real-time PCR and expressed sequence tags.

• 出版日期2010-5