Microfluidity mapping using fluorescence correlation spectroscopy: A new way to investigate plasma membrane microorganization of living cells

作者：Winckler Pascale; Cailler Aurelie; Deturche Regis; Jeannesson Pierre; Morjani Hamid; Jaffiol Rodolphe^*

来源：Biochimica et Biophysica Acta-Biomembranes, 2012, 1818(11): 2477-2485.

DOI：10.1016/j.bbamem.2012.05.018

• Fluorescence spectroscopy
• Fluorescence correlation spectroscopy
• Plasma membrane organization
• Microdomain
• Membrane fluidity

Diffusion time distribution analysis has been employed to highlight the microfluidity fingerprint of plasma membrane of living cells. Diffusion time measurements were obtained through fluorescence correlation spectroscopy performed at the single cell level, over various eukaryotic cell lines (MCF7, LR73, KB3.1, MESSA and MDCKII). The nonsymmetric profile of the diffusion time distributions established experimentally, is discussed according to Monte Carlo simulations, which reproduce the diffusion of the fluorescent probe in heterogeneous membrane.

• 出版日期2012-11
• 单位中国地震局