Antimicrobial resistance and integrons of ESBL-producing thermotolerant coliforms from a water reservoir in Tai'an, China

作者：Zhang, Hongna^*; Zhao, Xiaonan; Zhai, Zhenzhen; Li, Qing; Guo, Shuyuan; Chang, Weishan^*

来源：Journal of Infection in Developing Countries, 2017, 11(10): 740-746.

DOI：10.3855/jidc.8449

• Thermotolerant coliform
• integron
• water reservoir
• ESBL
• antibiotic resistance genes

Introduction: The contamination of water environments by extended-spectrum beta-lactamases-producing thermotolerant coliforms (ESBL-TC) has aroused public concern. However, little epidemiological data on ESBL-TC isolates from water reservoirs is available in China.
Methodology: This study was designed to investigate antibiotic resistance, bla gene types, and the presence of integrons (class 1, 2, and 3) and gene cassettes in ESBL-TC isolated from the Huangqian Reservoir of Tai'an, China.
Results: A total of 96 non-duplicate ESBL-TC were obtained in this study and the ESBL genes included blaCTX-M-14 (n = 47), blaCTX-M-15 (n = 27), blaCTX-M-55 (n = 18), blaSHV-12 (n = 4), blaCTX-M-3 (n = 3), and blaCTX-M-123 (n = 1). Eighty-three of the ninety-six ESBL-TC contained class 1 integrons (86.5%), and 2 isolates harbored class 2 integrons. The sizes of gene cassette regions within integrons were ranged from 0.2 kb to 3.2 kb.
Conclusions: The findings of this study indicated the widespread presence of ESBL-TC strains in the Huangqian Reservoir and spotlighted the potential role of water bodies as reservoirs for antibiotic resistant genes.

• 出版日期2017-10
• 单位河北大学; 泰安市中心医院; 河北经贸大学; 山东农业大学