Determination of sulfonamides in serum by on-line solid-phase extraction coupled to liquid chromatography with photoinduced fluorescence detection

作者：Arroyo Manzanares Natalia; Lara Francisco J; Airado Rodriguez Diego; Gamiz Gracia Laura; Garcia Campana Ana M^*

来源：Talanta, 2015, 138: 258-262.

DOI：10.1016/j.talanta.2015.03.012

• On-line solid-phase extraction
• Sulfonamides
• Liquid chromatography
• Photoinduced fluorescence
• Serum

An analytical method based on on-line solid-phase extraction coupled to liquid chromatography with photoinduced fluorescence detection has been developed to determine sulfonamides in serum. A home-made setup was used to percolate 3 mL of sample through a solid-phase extraction column. Analytes were retained onto the sorbent by an anion exchange mechanism which ensures an optimum compatibility with the subsequent chromatographic separation using a C-18 column and an on-line photoreactor in order to derivatize sulfonamides, which do not present native fluorescence. The method allowed the determination of 7 sulfonamides in serum samples previously deproteinized in less than 18 mm and with limits of detection ranging between 1.8 and 3.6 mg/L. Relative recoveries between 91.5% and 102.1% were obtained with satisfactory precision since relative standard deviations were always below 10.5%.

• 出版日期2015-6-1