ER-luminal thiol/selenol-mediated regulation of Ca2+ signalling

作者：Appenzeller Herzog Christian; Simmen Thomas

来源：Biochemical Society Transactions, 2016, 44(2): 452-459.

DOI：10.1042/BST20150233

• calcium
• endoplasmic reticulum (ER)
• inositol 1,4,5-trisphosphate receptor (IP3R)
• mitochondria-associated membrane (MAM)
• redox-dependent regulation
• sarco/endoplasmic reticulum Ca2+ transport ATPase (SERCA)
• signal transduction

The endoplasmic reticulum (ER) is the main cellular Ca2+ storage unit. Among other signalling outputs, the ER can release Ca2+ ions, which can, for instance, communicate the status of ER protein folding to the cytosol and to other organelles, in particular the mitochondria. As a consequence, ER Ca2+ flux can alter the apposition of the ER with mitochondria, influence mitochondrial ATP production or trigger apoptosis. All aspects of ER Ca2+ flux have emerged as processes that are intimately controlled by intracellular redox conditions. In this review, we focus on ER-luminal redox-driven regulation of Ca2+ flux. This involves the direct reduction of disulfides within ER Ca2+ handling proteins themselves, but also the regulated interaction of ER chaperones and oxidoreductases such as calnexin or ERp57 with them. Well-characterized examples are the activating interactions of Ero1 alpha with inositol 1,4,5-trisphosphate receptors (IP(3)Rs) or of selenoprotein N (SEPN1) with sarco/endoplasmic reticulum Ca2+ transport ATPase 2 (SERCA2). The future discovery of novel ER-luminal modulators of Ca2+ handling proteins is likely. Based on the currently available information, we describe how the variable ER redox conditions govern Ca2+ flux from the ER.

• 出版日期2016-4-15